Effects of urbanization and accessibility to sanitation services on water quality in urban streams in Uruguay.

The world's urban population is growing rapidly, and threatening natural ecosystems, especially streams. Urbanization leads to stream alterations, increased peak flow frequencies, and reduced water quality due to pollutants, morphological changes, and biodiversity loss, known as the urban stream syndrome. However, a shift towards recognizing urban streams as valuable natural systems is occurring, emphasizing green infrastructure and nature-based solutions. This study in Uruguay examined water quality in various watersheds with different urbanization levels and socio-environmental characteristics along a precipitation gradient. Using Geographic Information Systems (GIS) and in situ data, we assessed physicochemical parameters, generated territorial variables, and identified key predictors of water quality. We found that urbanization, particularly urban areas, paved areas, and populations without sanitation, significantly influenced water quality parameters. These factors explained over 50% of the variation in water quality indicators. However, the relationship between urbanization and water quality was non-linear, with abrupt declines after specific urban intensity thresholds. Our results illustrate that ensuring sanitation networks and managing green areas effectively are essential for preserving urban stream water quality. This research underscores the importance of interdisciplinary teams and localized data for informed freshwater resource management.

Erythrocyte Recovery in Oreochromis niloticus Fish Exposed to Urban Effluents.

Urban activities pollute aquatic ecosystems, and the integrity of organisms such as fish. The use of cytological techniques, such as the analysis of blood cellular integrity using the Micronucleus test, can help detect mutagenic damage as a result to urban effluents exposure. In this context, this study aimed to evaluate the frequency of micronucleus and other nuclear abnormalities in Oreochromis niloticus fish environmentally exposed to urban effluents in relation to their erythrocyte recovery capacity when exposed to clean water (30 and 45 days). The results indicated high copper, dissolved iron, nickel, and thermotolerant coliform levels in the urban stream. There was no difference in the frequency of micronuclei. In contrast, cells with nuclear nuclei, binucleates, kidney-shaped nuclei, notched nuclei, lobed nuclei, and segmented nuclei decreased according to the time the fish were exposed to clean water. When exposed to clean water, we conclude that urban fish recover from genotoxic and cytotoxic damage.

The impact of combined sewer outflows on urban water quality: Spatio-temporal patterns of fecal coliform in indianapolis.

Many urban waterways with older stormwater drainage systems receive a significant amount of untreated or poorly treated waste from Combined Sewer Outflow (CSO) systems during precipitation events. The input of effluent waste from CSO to urban water streams during storm events often leads to elevated fecal coliform, specifically Escherichia Coli (E. Coli) in these waterways. The aim of the study is to examine fecal coliform concentration, water chemistry, and water quality parameters to better understand spatio-temporal patterns of fecal coliform associated with CSO events in three waterways from Indianapolis, Indiana (USA). The waterways are Pleasant Run Creek (PRW), Fall Creek (FC) and White River (WR). The sampling occurred biweekly over one year for PRW, nine months for FC, and an intense (∼every three days) sub-analysis of the presumed peak period of fecal coliform growth (July) for WR. All PRW and FC sampling sites significantly exceeded the EPA contact standard limit of 200 CFU/100 mL for fecal coliform concentrations during the sampling period. We found no relationship between fecal coliform levels and the number or density of CSO outfalls above a given site. The most significant predictors of increased fecal coliform concentrations were precipitation on the sampling day and cumulative degree days. The most significant predictors of decreased fecal coliform were maximum precipitation during the ten-day window prior to sampling and median discharge during a three-day window prior to sampling. These findings suggest a push-pull balance within the system where CSO activation and seasonal gradients replenish and promote fecal coliform growth. At the same time, large hydrologic events act to flush and dilute fecal coliform concentrations. The results from this study help us to better understand how different drivers influence fecal coliform growth and how this information can be potentially used to predict and remediate the conditions of urban water streams.

Methane and nitrous oxide concentrations and fluxes from heavily polluted urban streams: Comprehensive influence of pollution and restoration.

Streams draining urban areas are usually regarded as hotspots of methane (CH4) and nitrous oxide (N2O) emissions. However, little is known about the coupling effects of watershed pollution and restoration on CH4 and N2O emission dynamics in heavily polluted urban streams. This study investigated the CH4 and N2O concentrations and fluxes in six streams that used to be heavily polluted but have undergone different watershed restorations in Southwest China, to explore the comprehensive influences of pollution and restoration. CH4 and N2O concentrations in the six urban streams ranged from 0.12 to 21.32 µmol L-1 and from 0.03 to 2.27 µmol L-1, respectively. The calculated diffusive fluxes of CH4 and N2O were averaged of 7.65 ± 9.20 mmol m-2 d-1 and 0.73 ± 0.83 mmol m-2 d-1, much higher than those in most previous reports. The heavily polluted streams with non-restoration had 7.2 and 7.8 times CH4 and N2O concentrations higher than those in the fully restored streams, respectively. Particularly, CH4 and N2O fluxes in the fully restored streams were 90% less likely than those found in the unrestored ones. This result highlighted that heavily polluted urban streams with high pollution loadings were indeed hotspots of CH4 and N2O emissions throughout the year, while comprehensive restoration can effectively weaken their emission intensity. Sewage interception and nutrient removal, especially N loadings reduction, were effective measures for regulating the dynamics of CH4 and N2O emissions from the heavily polluted streams. Based on global and regional integration, it further elucidated that increasing environment investments could significantly improve water quality and mitigate CH4 and N2O emissions in polluted urban streams. Overall, our study emphasized that although urbanization could inevitably strengthen riverine CH4 and N2O emissions, effective eco-restoration can mitigate the crisis of riverine greenhouse gas emissions.

Urban stream syndrome: Quantifying topographic variation along an urban-rural gradient.

Topographic variation within fluvial systems is essential for providing a mosaic of physical habitats and supporting the dynamic hydraulic, geochemical, and biological processes that determine both aquatic and riparian ecosystem function. In highly-modified rivers through both urban and rural settings, the physical heterogeneity of alluvial channels has been diminished by anthropogenic activities. As riparian areas are increasingly under pressure from agricultural and urban development, identifying the geomorphic controls on physical heterogeneity through these environments is critical. In this study, we use the bed coefficient of variation (CV) extracted from a high-resolution bathymetric LiDAR survey as a dimensionless metric for topographic variation and physical heterogeneity over 100 km of the Boise River corridor that spans an urban-rural gradient. Our CV results for both the streambed and channel demonstrate that the average topographic variation of reaches in urban areas is 22-25% lower than reaches located in rural areas along the same river. While these results initially support the application of the urban stream syndrome hypothesis, CV values had similar magnitudes in both urban and rural reaches suggesting there is a dominant control on topographic variation that was not directly related to urban land use. Analysis of CV values relative to normalized levee width indicates that the causative driver of morphologic simplification in the channel was lateral constraints from levees. In the Boise River, topographic variation increased linearly with normalized levee widths that ranged between 50% and >300% of the average channel width. Further, topographic variation was maximized in reaches where flow expansion during high discharge inundated between 1 and 2 times the average channel width (approximately 65-70% of the available floodplain). Our simple and objective watershed-scale approach leverages high-resolution topography data to identify reaches of high physical heterogeneity for river conservation, as well as help guide environmental flow releases in managed rivers.

Long-term assessment of floodplain reconnection as a stream restoration approach for managing nitrogen in ground and surface waters.

Stream restoration is a popular approach for managing nitrogen (N) in degraded, flashy urban streams. Here, we investigated the long-term effects of stream restoration involving floodplain reconnection on riparian and in-stream N transport and transformation in an urban stream in the Chesapeake Bay watershed. We examined relationships between hydrology, chemistry, and biology using a Before/After-Control/Impact (BACI) study design to determine how hydrologic flashiness, nitrate (NO3 -) concentrations (mg/L), and N flux, both NO3 - and total N (kg/yr), changed after the restoration and floodplain hydrologic reconnection to its stream channel. We examined two independent surface water and groundwater data sets (EPA and USGS) collected from 2002-2012 at our study sites in the Minebank Run watershed. Restoration was completed during 2004 and 2005. Afterward, the monthly hydrologic flashiness index, based on mean monthly discharge, decreased over time from 2002 and 2008. However, from 2008-2012 hydrologic flashiness returned to pre-restoration levels. Based on the EPA data set, NO3 - concentration in groundwater and surface water was significantly less after restoration while the control site showed no change. DOC and NO3 - were negatively related before and after restoration suggesting C limitation of N transformations. Long-term trends in surface water NO3 - concentrations based on USGS surface water data showed downward trends after restoration at both the restored and control sites, whereas specific conductance showed no trend. Comparisons of NO3 - concentrations with Cl- concentrations and specific conductance in both ground and surface waters suggested that NO3 - reduction after restoration was not due to dilution or load reductions from the watershed. Modeled NO3 - flux decreased post restoration over time but the rate of decrease was reduced likely due to failure of restoration features that facilitated N transformations. Groundwater NO3 - concentrations varied among stream features suggesting that some engineered features may be functionally better at creating optimal conditions for N retention. However, some engineered features eroded and failed post restoration thereby reducing efficacy of the stream restoration to reduce flashiness and NO3 - flux. N management via stream restoration will be most effective where flashiness can be reduced and DOC made available for denitrifiers. Stream restoration may be an important component of holistic watershed management including stormwater management and nutrient source control if stream restoration and floodplain reconnection can be done in a manner to resist the erosive effects of large storm events that can degrade streams to pre-restoration conditions. Long-term evolution of water quality functions in response to degradation of restored stream channels and floodplains from urban stressors and storms over time warrants further study, however.

Occurrence and accumulation of pharmaceutical products in water and biota of urban lowland rivers.

We evaluated the distribution of eleven groups of pharmaceutically active compounds (PhACs) in surface waters and biota of different trophic levels, in five sites of two lowland urban rivers in Argentine. Twenty-nine out of 39 PhACs and two metabolites were detected in at least one water sample (2-9622 ng/L), eleven detected in biofilms (1-179 ng/g d.w.) and eight in the macrophyte Lemna gibba (4-112 ng/g d.w). The two more polluted sites had a similar distribution of the main groups of compounds. In surface waters, the largest concentrations were for the analgesic acetaminophen (9622 ng/L), the antibiotic sulfamethoxazole (326 ng/L), the antihypertensive valsartan (963 ng/L), the ß-blocking agent atenolol (427 ng/L), the diuretic hydrochlorothiazide (445 ng/L) and the psychiatric drug carbamazepine (99 ng/L). The antibiotic ciprofloxacin exhibited the highest concentration in the biofilm (179 ng/g d.w.) and in the macrophyte L. gibba (112 ng/g d.w.) Several compounds were detected in the water but not in the biota (e.g., codeine and bezafibrate), and others (e.g., azithromycin and citalopram) were found in the biota but not in the surface water. Significant bioaccumulation factors (>1000 L/kg d.w.) were obtained for venlafaxine and ciprofloxacin in biofilm. Our results show that PhACs may accumulate in several biological compartments. Within an environmental compartment, similar PhACs profile and concentrations were found in different sites receiving urban pollution. Among different compartments, biofilms may be the most suitable biota matrix to monitor the immediate reception of PhACs in the biota. Our results indicate that the presence of PhACs in urban rivers and their accumulation in the biota could be incorporated as symptoms of the urban stream syndrome.

Characterizing the Chemical Profile of Biological Decline in Stormwater-Impacted Urban Watersheds.

Chemical contamination is an increasingly important conservation issue in urban runoff-impacted watersheds. Regulatory and restoration efforts typically evaluate limited conventional parameters and pollutants. However, complex urban chemical mixtures contain hundreds to thousands of organic contaminants that remain unidentified, unregulated, and poorly understood. This study aimed to develop broadly representative metrics of water quality impairment corresponding to previously documented biological degradation along gradients of human impacts. Stream samples (n = 65, baseflow/rainfall conditions, 2017-2018) were collected from 15 regional watersheds (Puget Sound, WA, USA) across an urbanization gradient defined by landscape characteristics. Surface water chemical composition characterized via non-targeted high-resolution mass spectrometry (7068 detections) was highly correlated with landscape-based urbanization gradient (p < 0.01) and season (p < 0.01). Landscape-scale changes in chemical composition closely aligned with two anchors of biological decline: coho salmon (Oncorhynchus kisutch) mortality risk (p < 0.001) and loss of stream macroinvertebrate diversity and abundance (p < 0.001). We isolated and identified 32 indicators for urban runoff impacts and corresponding receiving water ecological health, including well-known anthropogenic contaminants (e.g., caffeine, organophosphates, vehicle-derived chemicals), two related environmental transformation products, and a novel (methoxymethyl)melamine compound. Outcomes support data-directed selection of next-generation water quality indicators for prioritization and evaluation of watershed management efforts intended to protect aquatic ecosystems.

Database of fish fauna in a highly urbanised river (Tsurumi River Basin, Kanagawa, Japan).

Background: Urbanisation has facilitated changes in the hydrological cycle, sediment dynamics and habitat loss and it has had a tremendous impact on river ecosystems. Moreover, the invasion of non-native species reduces the number of native species with the progress of urbanisation, thereby resulting in the homogenisation of fish fauna and significant decrease in diversity. However, the effects of urbanisation on fish fauna vary depending on the region, degree of urbanisation and number of years since the construction of the city. Hence, it is necessary to collect information on how fish fauna changes due to urbanisation in different regions. The target site of the present study is the Tsurumi River, of which approximately 85% of the basin is urbanised and many of the river channels have been affected by straightening and concrete channelling. Monitoring of biota has been conducted mainly in downstream areas; however, data on fish fauna in upstream areas and tributaries of the river, which have been substantially affected by urbanisation, are lacking. New information: Surveys were conducted at 71 sites in the Tsurumi River Basin during summers and winters, focusing on river channels that have been straightened or converted to concrete channels due to urbanisation. As a result of this investigation, 10 families, 28 species and 9,335 individuals were collected. Some of the fish collected included rare species, such as Tanakialanceolata, Lefuaechigonia and Pseudobagrustokiensis, indicating that, even in rivers that have been severely impacted by human activities, there are still rare indigenous species living there, albeit in limited numbers. In addition, Misgurnus sp. (clade B2), Micropterussalmoides, Lepomismacrochirus and Poeciliareticulata were confirmed to be non-native species. Misgurnus sp. (clade B2) was widespread; however, populations of carnivorous non-native alien species were small. The data are all accessible from the document "database_fish_urban_tsurumi" (https://ipt.pensoft.net/manage/resource?r=database_fish_urban_tsurumi).

Small stream catchments in a developing city context: The importance of land cover changes on the ecological status of streams and the possibilities for providing ecosystem services.

Urban expansion and the transformation of rural areas into suburban areas along with increasing human pressure can cause major changes in the environment. Typical effects of urbanization include degradation of aquatic ecosystems. However, urban expansion is often accompanied by an effort to provide residents with some contact with nature in the city. Natural waterbodies are the elements of the environment predestined to ensure appropriate conditions for recreation and other ecosystem services key to human well-being. In this study, the changes in the forms of development of a submontane stream catchment area (Carpathians, Poland) against the background of the gradual extension of city boundaries were analysed. In addition, the impact of urbanization on the ecological conditions of the urban stream, and therefore the potential ecosystem to provide ecosystem services, was assessed. Although the extension of the city entailed the development of settlements, it was accompanied by the expansion of the municipal sewage infrastructure, thus improving sewage management. The disappearance of arable lands from the stream catchment due to the decline in agricultural activities initiated the spontaneous process of buffer restoration along the stream. All the biotic indices such as Shannon diversity index, Margalef species richness index and BMWP-PL index, based on macroinvertebrate communities increased, and the fish were more numerous in the late stage of city development compared with the early (rural) stage. Surprisingly, the system has rehabilitated well with no revitalisation actions. It is necessary, however, to improve morphological in-stream conditions, and to make further efforts to improve water quality for the system to develop a semi-natural state. Only reaching full ecological potential can lead to an increase in the ecosystem capacity to provide ecosystem services. The studied ecosystem may provide many different services simultaneously, but their availability depends on the protection of the ecosystem's functioning as a whole.

Groundwater-surface water interactions and flux of organic matter and nutrients in an urban, Mediterranean stream.

The chemical quality of dissolved organic matter (DOM) and the speciation of nitrogen exported from urban catchments is of great importance to biogeochemical cycling in riverine and coastal receiving waters. Many urban streams in Mediterranean climates have a flashy hydrologic regime, which would suggest a rapid pulsing and shunting of solutes downstream. However, the role of these systems both as passive pipes for solute transport or as reactors for DOM and nutrient transformation is still an open question for urban, Mediterranean streams. To address this question, we evaluated changes in concentrations of inorganic and organic solutes and DOM optical properties in Alvarado Creek, a perennially-flowing, urban, first-order tributary of the San Diego River in San Diego, CA, USA, during dry weather (baseflow) conditions and during four storm events in 2016-2018. Chloride and sulfate concentrations corroborate the supposed saline groundwater supply that maintains perennial flow and brackish nature in this urban stream. During dry weather, high proportions of protein-like fluorescent component (AC4) and downstream decreases in total dissolved nitrogen (TDN) and nitrate imply in-stream processing (nitrification and denitrification). By contrast, storm hysteresis curves indicate that the supply of DOM and TDN was not exhausted over the duration of a storm event, whereas nitrate was eventually depleted, presumably because nitrification could not keep up with the export of nitrate from source areas. Rapid decreases in chloride during the storm hydrograph coincided with a shift in specific ultraviolet absorbance (SUVA) and fluorescence index (FI) to more terrestrially-derived and aromatic carbon sources, most likely from interflow of stormwater through vadose zone soils. On an annual basis, the export of microbially-derived DOM during dry weather was higher than the export of terrestrially-derived DOM during storm events; both represent important carbon inputs to coastal waters.

Identifying Sources of Faecal Contamination in a Small Urban Stream Catchment: A Multiparametric Approach.

Small urban streams discharging in the proximity of bathing waters may significantly contribute to the deterioration of water quality, yet their impact may be overlooked. This study focuses on the Elm Park stream in the city of Dublin that is subject to faecal contamination by unidentified sources. The aim of the study was to identify a minimum number of "sentinel" sampling stations in an urban catchment that would provide the maximum amount of information regarding faecal pollution in the catchment. Thus, high-resolution sampling within the catchment was carried out over the course of 1 year at 11 stations. Faecal indicator bacteria were enumerated and microbial source tracking (MST) was employed to evaluate human pollution. In addition, ammonium, total oxidised nitrogen, and phosphorus levels were monitored to determine if these correlated with faecal indicator and the HF183 MST marker. In addition, the effect of severe weather events on water quality was assessed using automated sampling at one of the identified "sentinel" stations during baseflow and high flow conditions over a 24-h period. Our results show that this urban stream is at times highly contaminated by point source faecal pollution and that human faecal pollution is pervasive in the catchment. Correlations between ammonium concentrations and faecal indicator bacteria (FIB) as well as the human MST marker were observed during the study. Cluster analysis identified four "sentinel" stations that provide sufficient information on faecal pollution in the stream, thus reducing the geographical complexity of the catchment. Furthermore, ammonium levels strongly correlated with FIB and the human HF183 MST marker under high flow conditions at key "sentinel" stations. This work demonstrates the effectiveness of pairing MST, faecal indicators, and ammonium monitoring to identify "sentinel" stations that could be more rapidly assessed using real-time ammonium readouts to assess remediation efforts.

Simple screening of microplastics in bottled waters and environmental freshwaters using a novel fluorophore.

Screening of polymeric microplastic debris can help to assess the extent to which plastics contaminate the environment. We here developed an easy- and rapid-to-perform method for the screening of plastic polymers, based on a newly employed fluorophore, namely 1-pyrenebutyric acid N-hydroxysuccinimidyl ester (PBN). The PBN fluorophore was capable of staining diverse synthetic microplastic polymers within 5 min, including those displaying various particle sizes and shapes. The fluorescence intensities of the microplastics were considerably enhanced after the short-duration staining. The screening method was shown to be highly effective in the detection of polyethylene (PE), poly(ethylene terephthalate) (PET), polyvinyl chloride (PVC), polyamide-6 (PA-6), polypropylene (PP), polystyrene (PS), polycarbonate (PC), polyvinylidenechloride (PVDC), and polyurethane (PU), with a lowest analyzed particle size of 15 µm. Using our screening method, plastic contamination was investigated in commercially available bottled waters and environmental waters, specifically urban freshwaters. This study demonstrated high affinity levels of the newly proposed PBN fluorophore for a broad range of polymers and its ability to be used to discernibly identify polymer particles.

The Urban River Syndrome: Achieving Sustainability Against a Backdrop of Accelerating Change.

Human activities have been affecting rivers and other natural systems for millennia. Anthropogenic changes to rivers over the last few centuries led to the accelerating state of decline of coastal and estuarine regions globally. Urban rivers are parts of larger catchment ecosystems, which in turn form parts of wider nested, interconnected systems. Accurate modelling of urban rivers may not be possible because of the complex multisystem interactions operating concurrently and over different spatial and temporal scales. This paper overviews urban river syndrome, the accelerating deterioration of urban river ecology, and outlines growing conservation challenges of river restoration projects. This paper also reviews the river Thames, which is a typical urban river that suffers from growing anthropogenic effects and thus represents all urban rivers of similar type. A particular emphasis is made on ecosystem adaptation, widespread extinctions and the proliferation of non-native species in the urban Thames. This research emphasizes the need for a holistic systems approach to urban river restoration.

Polycyclic aromatic hydrocarbons (PAHs) in urban stream sediments of Suzhou Industrial Park, an emerging eco-industrial park in China: Occurrence, sources and potential risk.

In this study, urban stream sediment samples were collected in the Suzhou Industrial Park (SIP), one of the earliest national demonstration eco-industrial parks of China. PAHs were analyzed in these sediments, and concentrations of total PAHs were 180-81,000 ng g-1 (5700 ± 14,000 ng g-1). Medium molecular weight (4- ring) PAHs were predominant (42 ± 12%), followed by high molecular weight (5- and 6- ring) PAHs (31 ± 10%). No correlation was found between concentrations of PAHs and land uses of SIP in this study. Diagnostic ratios and a positive matrix factorization (PMF) model indicated that coal/biomass combustion might be the primary PAH source (61%), followed by non-combustion sources (21%) and vehicular emission (18%). According to the spatial analysis, PAHs in the sediments of SIP might be mainly associated with the coal/biomass combustion in the northeast industrial zone. Residential & commercial activities seem not to be the major causes of PAH contamination. Total PAH toxic equivalent concentrations, effect range low/effect range median values, and mean effects range-median quotient all showed that PAHs were present at a low toxicity risk level in most regions of the SIP. However, vigilance is required at some sampling sites with extremely high PAH concentrations or high mean effects range-median quotient.

Seasonal variation of Hedruris dratini (Nematoda) parasitizing Hydromedusa tectifera (Chelidae), with focus on host's torpor state.

BACKGROUND: The aim of this study was to analyze the seasonal distribution of the nematode Hedruris dratini parasitizing the South American Snake-necked turtle Hydromedusa tectifera and the amphipod Hyalella spp. in an urban stream. We focused on understand which strategies parasite population displays to get through the host's hibernation period. RESULTS: The highest prevalence and abundance of H. dratini were found in summer. The parasitic load was lower in winter, however there were no significant differences when it was compared with autumn and spring. Generalized linear model identified the temperature as a determining factor for the presence of parasites in turtles. CONCLUSIONS: Our results indicate that, beside turtles enter in a diapause state, the life cycle of H. dratini never stop throughout the year, being a continuous transmission between both the intermediate and final host throughout the year. Turtles feed and become infected with parasite larvae even in winter although with a lower ingestion rate.

Percepción de dos comunidades sobre la contaminación del arroyo el Riíto, Chiapas, México / Perception of two communities of the pollution about the el Riito stream, Chiapas, Mexico

Resumen: El presente artículo identificó y evaluó, mediante la percepción ambiental con enfoque geográfico (PAEG), los impactos sociales y ambientales a nivel de comunidades derivados de la contaminación presente en el arroyo denominado el Riíto en Tonalá, Chiapas. Para ello, se aplicó una metodología mixta que comprendió la identificación de actores sociales, entrevistas y aplicación de encuestas estructuradas mediante procedimientos estadísticos en dos comunidades de la costa de Chiapas: Paredón y Tonalá. Este tipo de investigación, además, permitió identificar debilidades en la gobernanza y gestión de las instituciones presentes en la zona. Se evidenció que no logran dar una solución integral a un problema de agua que pone en riesgo la salud de las dos comunidades en estudio y mucho menos al medio ambiente de la microcuenca.

Abstract: This article identified and evaluated through Environmental Perception with a Geographical Approach (PAEG), the social and environmental impacts at the community level derived from the contamination present in the stream called El Riíto in Tonalá, Chiapas. For this, a mixed methodology was applied, which included from the identification of social actors, interviews and the application of structured surveys using statistical procedures in two communities on the Chiapas coast: Paredón and Tonalá. This type of research also allowed identifying weaknesses in the governance and management of the institutions present in the area, as they fail to provide a comprehensive solution to a water problem that puts the health of the two communities under study at risk, much less to the micro basin environment.

Water quality evaluation of two urban streams in Northwest Uruguay: are national regulations for urban stream quality sufficient?

Poor water quality in urban streams places at risk the health of urban residents and the integrity of urban environments, ultimately leading to the "urban stream syndrome." In response to growing concerns regarding urban streams in Uruguay, we evaluated the spatiotemporal variation in water quality parameters in two urban streams, the Ceibal and La Curtiembre streams, over 12-18 months. A proposal for an Urban Water Quality Index (UWQI) was developed based on national water quality standards for two stream classes to assess variability in overall stream condition over time. Both streams displayed extremely high levels of fecal coliform concentrations in mid-downstream sites and relatively high levels of total dissolved phosphorus and ammonia well above the national standards of 0.025 mg/L and 0.02 mg/L, respectively. Nitrate was consistently below the national maximum of 44.3 mg/L, calling to question the adequacy of this standard for designated uses. Over 40% of samples had oxygen levels below the national standard of 5 mg/L, and a dead zone (average 1.5 mg/L) was detected in the downstream reach of the La Curtiembre stream. Despite differences in land use and urban context, monthly observations of nutrients and coliforms indicated high levels of contamination in mid-downstream reaches, which could present a health risk for the populations in Paysandú and Salto. This study highlights the degradation of urban streams in two major cities in Uruguay and the need for a comparative diagnosis of stream condition as a basis for decision-making regarding urban development and water resources.

Correlation between antimicrobial resistance and faecal contamination in small urban streams and bathing waters.

Antibiotic resistance represents the greatest challenge to healthcare systems around the world. As antibiotic resistance genes (ARGs) are shed in faeces, many studies have focused on how wastewater effluent contributes to ARG pollution in rivers. However, small urban streams and bathing waters not impacted by treated wastewater have received little attention though they may be important reservoirs of ARGs. The main objective of this study was to assess the extent to which ARG and faecal pollution impact small urban streams and bathing waters and to determine if there is a relationship between these contaminants. For one year, bi-monthly water samples were collected from two urban streams and Dublin city's three designated bathing waters. The Liffey Estuary, that receives treated wastewater, was also sampled. The sul1, tet(O), qnrS, blaTEM, blaSHV and blaCTX-M ARGs were quantified. E. coli and intestinal enterococci levels were determined and the source of faecal pollution (human, dog, gull) quantified by microbial source tracking. Our results show that the Liffey Estuary, the urban streams and the bathing waters are highly impacted by ARGs and human faeces. There were clear correlations between all of the studied faecal indicators and ARGs in the Liffey Estuary. In the urban streams relationships were observed for only some of the ARGs and faecal indicators, which is likely a result of non-continuous sewage leaks and overflows to the streams. Similarly, only some ARGs correlated with faecal indicators in the urban bathing waters. The source of ARGs in the bathing waters is likely to be multifaceted as we detected sporadic dog and gull faecal markers. This study demonstrates that small urban streams and bathing waters are reservoirs of ARGs and that they may pose a previously unrecognised public health risk as they have the potential to transmit enteric pathogens and antibiotic resistance determinants.

Urbanization can increase the invasive potential of alien species.

Alien species often flourish and become invasive in urban ecosystems. How and why invaders succeed in urban systems is an important, yet poorly understood, question. We investigate whether the success of urban invaders is related to changes in species traits that enhance invasive potential. We also explore whether a trophic mechanism helps explain the success of invaders in urban systems. We use the guppy Poecilia reticulata, a globally distributed alien species that has invaded both urban and non-urban systems, as our model. We first characterize the effect of urbanization on streams where guppies are present. We measure guppy invasion success using their population density and size-frequency. Then we assess how traits that are related to the potential of guppies to invade (life history and condition) respond to urbanization. Next, we explore how urbanization affects the availability of food for guppies and their diets. We also test if the presence of other fish species grants biological resistance to invasion by dampening guppy invasive potential. We find that urban streams have high concentrations of ammonium and faecal coliforms, indicating contamination from sewage. On average, guppy populations from urban streams have 26× higher density and larger body sizes than non-urban populations. Urban guppies are in better condition and have on average five more offspring than non-urban guppies. Urbanization increases the availability and consumption of highly nutritious food (chironomid larvae) by guppies. We find a positive relationship between the consumption of chironomids and both fecundity and condition. The presence of other fish species in urban streams often has a negative but small effect on guppy traits and density. Our data suggest a relaxation of trade-offs that shape life-history traits which is related to increased food resources in urban streams. These indicate that urbanization enhances the invasive potential of guppies through a trophic mechanism that simultaneously increases reproduction and somatic investment. Such mechanism is likely widespread because chironomids are often highly abundant in urban systems. Thus, not only guppies but also other invasive species can take advantage of such a resource to invest in traits that enhance invasion success.